Biology Board Question Paper Solution
Maharashtra State Board - March 2020 (HSC Class 12)
Max. Marks: 70 | Time: 3 Hours
(Reason: Chromatium is a photoautotroph, while others are chemoautotrophs.)
HSC Biology
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i. Where were the bones of jaws and teeth of Ramapithecus found?
ii. In electrocardiogram, QRS complex stands for:
iii. Laxman has low secretion of ADH resulting in _______ type of diabetes.
iv. Name the region of retina where rods and cones are absent.
v. Among biotic components, the micro consumers are called _______.
vi. Identify ‘A’ in the chart given below:
| Product | Plant |
|---|---|
| (1) Nicotine | Nicotiana tabacum |
| (2) Vincristin, Vinblastin | ‘A’ |
vii. The genotypic ratio 1:2:2:4:1:2:1:2:1 is obtained in F2 generation. What will be the phenotypic ratio?
viii. Define the term ‘recessive’.
Attempt any eight of the following questions:
(Student should draw the 7-celled, 8-nucleate structure of the female gametophyte)
Labels required:
- Chalazal end: Contains 3 Antipodal cells.
- Central part: Large central cell with Secondary nucleus (or two Polar nuclei).
- Micropylar end: Egg apparatus containing 1 Egg cell (Oosphere) and 2 Synergids.
- Filiform apparatus: Inside synergids.
C4 plants show a specialized anatomy called Kranz anatomy to avoid photorespiration:
- Dimorphic Chloroplasts:
- Mesophyll cells: Contain granal chloroplasts (with grana).
- Bundle Sheath cells: Contain large, agranal chloroplasts (without grana).
- Concentric Arrangement: The bundle sheath cells form a wreath-like (Kranz) layer around the vascular bundles, surrounded by mesophyll cells.
- Thick Walls: Bundle sheath cells have thick walls impervious to gaseous exchange, concentrating CO2 internally.
- Isolation of DNA (Genetic material) from the donor organism.
- Cutting of DNA at specific locations using Restriction Endonuclease enzymes.
- Amplification of the gene of interest using PCR (Polymerase Chain Reaction).
- Insertion of the Recombinant DNA (rDNA) into the host cell/organism using a vector.
- Selection and screening of transformed host cells.
- Obtaining the foreign gene product (downstream processing).
i. Bio-patent
ii. Bio-piracy
i. Bio-patent: It is a patent granted by the government to the inventor for biological entities (like strains of microorganisms, cell lines, genetically modified strains), DNA sequences, and biotechnological processes and products.
ii. Bio-piracy: It refers to the use of bio-resources by multinational companies and other organizations without proper authorization from the countries and people concerned without compensatory payment.
It can also be represented including Replication:
We can identify the genotype of an F1 hybrid by performing a Test Cross. In a test cross, the F1 individual is crossed with the homozygous recessive parent.
Example: Consider height in pea plants (T = Tall, t = Dwarf).
- Case 1 (Heterozygous): If F1 is Hybrid Tall (Tt):
Cross: Tt (F1) × tt (Recessive parent)
Progeny: 50% Tall (Tt) and 50% Dwarf (tt). Ratio 1:1.
Conclusion: F1 is Heterozygous. - Case 2 (Homozygous): If F1 were Homozygous Tall (TT):
Cross: TT × tt
Progeny: 100% Tall (Tt).
Conclusion: F1 is Homozygous.
(Any two from the seven pairs)
- Stem height: Tall vs. Dwarf
- Seed colour: Yellow vs. Green
- Seed shape: Round vs. Wrinkled
- Pod colour: Green vs. Yellow
| Column I | Column II |
|---|---|
| (1) Mechanical means | (a) Saheli |
| (2) Physiological device | (b) Jellies |
| (3) Chemical device | (c) Vasectomy |
| (4) Permanent Method | (d) Diaphragm |
- (1) Mechanical means — (d) Diaphragm
- (2) Physiological device — (a) Saheli (Oral Contraceptive Pill)
- (3) Chemical device — (b) Jellies (Spermicides)
- (4) Permanent Method — (c) Vasectomy
Simplified Exam Diagram
Note: Students are required to draw this in the exam.
Detailed Diagram (Reference Only)
Note: For understanding purposes only. Not required for the exam.
The student needs to draw the transverse section of the spinal cord showing the reflex path. Key labels to include:
- Receptor: Skin (where pin prick occurs).
- Sensory Neuron (Afferent): Enters via Dorsal root.
- Dorsal Root Ganglion: Swelling on dorsal root containing cell body of sensory neuron.
- Association Neuron (Interneuron): Inside the Grey matter of spinal cord.
- Motor Neuron (Efferent): Leaves via Ventral root.
- Effector: Muscle (showing contraction).
Arrows should indicate flow: Skin \(\rightarrow\) Sensory Neuron \(\rightarrow\) Spinal Cord \(\rightarrow\) Motor Neuron \(\rightarrow\) Muscle.
Principle: It states that allele frequencies in a population remain constant from generation to generation in the absence of other evolutionary influences (like mutation, selection, migration).
The sum of allelic frequencies is 1: \(p + q = 1\)
The genotypic frequencies are given by: \((p + q)^2 = p^2 + 2pq + q^2 = 1\)
Punnett Square:
| Gametes | p (Dominant allele) | q (Recessive allele) |
|---|---|---|
| p | \(p^2\) (AA - Homozygous Dominant) | \(pq\) (Aa - Heterozygous) |
| q | \(pq\) (Aa - Heterozygous) | \(q^2\) (aa - Homozygous Recessive) |
| S.NO | Type | Example |
|---|---|---|
| 1. | Vulnerable species | Clouded leopard, Musk deer |
| 2. | ________________ | Great Indian Bustard, Hawaiian monk seal |
| 3. | ________________ | Three banded armadillo (Brazil), Short eared rabbit (Sumatra) |
- (Given) Vulnerable species
- Endangered species
- Intermediate species (Note: According to Maharashtra Board Textbook context)
(A) Fine particles | (B) Coarse particles
(A) Fine particles:
- Size: Less than 5 µm (or 2.5 µm depending on specific text edition) in diameter.
- Ex: (i) Aerosols
- (ii) Smoke / Soot / Fumes
(B) Coarse particles:
- Size: Over 5 µm in diameter.
- Ex: (i) Carbon particles
- (ii) Dust
Attempt any EIGHT of the following questions:
(i) Mechanoreceptors
(ii) Statoacoustic receptors
(iii) Baroreceptors
- (i) Mechanoreceptors:
Location: Skin.
Function: Detect mechanical stimuli like touch, pressure, and pain. - (ii) Statoacoustic receptors:
Location: Inner ear (Internal ear).
Function: Hearing (Phonoreceptors) and Body Balance/Equilibrium (Statoreceptors). - (iii) Baroreceptors:
Location: Walls of carotid sinus and aortic arch.
Function: Detect changes in blood pressure.
| Column A | Column B |
|---|---|
| (1) Plasma Proteins | (i) Serum albumin, (v) Fibrinogen |
| (2) Nitrogenous waste | (iii) Urea, (vi) Uric acid |
| (3) Inorganic Salts | (ii) Bicarbonates, (iv) Sulphates of sodium |
Causative agent: Protozoan parasite of the genus Plasmodium (e.g., Plasmodium vivax, P. falciparum).
Symptoms (Any two):
- High fever with chills and shivering.
- Severe headache and nausea.
- Profuse sweating followed by lowering of temperature.
Preventive measures (Any two):
- Use of mosquito nets and insect repellents to avoid bites.
- Elimination of mosquito breeding grounds (stagnant water).
- Spraying insecticides to kill adult mosquitoes and larvae.
[Diagram of Vaccine Production]
Write in brief about production of vaccine.
Identification:
- 1: Isolation of Antigen (Separation of specific antigen from the pathogen).
- 2: Formulation / Mixing (Mixing of antigen with diluent/adjuvant).
(Note: Interpretation based on standard vaccine production flowchart found in textbooks where step 1 is antigen isolation and step 2 is formulation).
Brief about production of vaccine:
Vaccines are produced using biotechnology. The pathogen is cultured and inactivated or attenuated. The specific antigen (protein) responsible for immunity is isolated ('1'). It is then mixed with a suitable diluent or adjuvant ('2') to increase stability and immune response. This mixture forms the final vaccine.
Analysis: Colorblindness is an X-linked recessive disorder.
- Satish is colorblind (\(X^cY\)).
- Maternal Grandfather was colorblind (\(X^cY\)). He passed his \(X^c\) chromosome to his daughter (Satish's mother).
- Satish's Mother is phenotypically normal but must be a carrier (\(X^CX^c\)) because she received the affected X from her father.
- Satish's Father is normal (\(X^CY\)).
Inheritance Chart:
Parents: Carrier Mother (\(X^CX^c\)) × Normal Father (\(X^CY\))
| Gametes | \(X^C\) (Sperm) | Y (Sperm) |
|---|---|---|
| \(X^C\) (Egg) | \(X^CX^C\) (Normal Daughter) | \(X^CY\) (Normal Son) |
| \(X^c\) (Egg) | \(X^CX^c\) (Carrier Daughter) | \(X^cY\) (Colorblind Son - Satish) |
Pattern of Inheritance: This is an example of Criss-cross inheritance. The gene for colorblindness was passed from the maternal grandfather to his daughter (carrier), and then from the daughter to her son (Satish).
Requirements of dairy management:
- Selection of good breeds with high yielding potential and disease resistance.
- Proper housing (well-ventilated, sufficient water).
- Scientific feeding (fodder quantity and quality).
- Hygiene and cleanliness during milking and handling.
- Regular veterinary checkups.
Examples:
- Indian breed: Sahiwal, Gir, or Red Sindhi.
- Exotic breed: Jersey, Holstein-Friesian, or Brown Swiss.
| Feature | DNA (Deoxyribonucleic Acid) | RNA (Ribonucleic Acid) |
|---|---|---|
| Sugar | Contains Deoxyribose sugar. | Contains Ribose sugar. |
| Strands | Usually double-stranded (Double Helix). | Usually single-stranded. |
| Nitrogen Bases | Contains Adenine, Guanine, Cytosine, and Thymine. | Contains Adenine, Guanine, Cytosine, and Uracil. |
| Function | Stores genetic information. | Helps in protein synthesis. |
Green Revolution: It refers to the drastic increase in the production of food grains (especially wheat and rice) in developing countries due to the introduction of high-yielding varieties (HYV), use of fertilizers, pesticides, and better irrigation techniques.
Examples:
- Wheat: Sonalika, Kalyan Sona.
- Rice: Jaya, Ratna (or Padma).
(i) Vitamin B12
(ii) Chloromycetin
(iii) Pectinase
- (i) Vitamin B12: Pseudomonas denitrificans (or Propionibacterium shermanii)
- (ii) Chloromycetin (Antibiotic): Streptomyces venezuelae
- (iii) Pectinase (Enzyme): Aspergillus niger (or Sclerotinia libertiana)
- Energy Release: It releases energy in the form of ATP, which is essential for various metabolic activities of the cell.
- Intermediates: It provides carbon skeleton intermediates required for the synthesis of other biomolecules (like amino acids, fatty acids).
- Substrate Activation: It converts insoluble complex food substances into soluble simpler forms.
- CO2 Balance: It releases CO2, which is used in photosynthesis, helping maintain the balance of gases in the atmosphere.
Anaerobic respiration occurs in the absence of oxygen. It involves two main steps:
- Glycolysis (EMP Pathway):
- Glucose (6C) is broken down into two molecules of Pyruvate (3C).
- Net gain: 2 ATP and 2 NADH2.
- This occurs in the cytoplasm.
- Fermentation:
- The pyruvate produced is reduced to other products depending on the organism.
- Alcoholic Fermentation (in Yeast): Pyruvate \(\rightarrow\) Acetaldehyde + CO2 \(\rightarrow\) Ethanol (Ethyl Alcohol). NADH2 is reoxidized to NAD.
- Lactic Acid Fermentation (in Muscle/Bacteria): Pyruvate \(\rightarrow\) Lactic Acid.
Overall, it produces very less energy (2 ATP) compared to aerobic respiration.
Citizens play a crucial role in solid waste management by adopting the following practices:
- 3R Principle: Following Reduce, Reuse, and Recycle to minimize waste generation.
- Segregation: separating waste into biodegradable (wet) and non-biodegradable (dry) waste at the source.
- Composting: Using wet waste (kitchen scraps) to make compost for home gardens.
- Avoiding Plastics: Reducing the use of single-use plastics and carrying cloth bags.
- Safe Disposal: Not littering in public places and disposing of hazardous waste (batteries, medicines) separately.
Attempt any THREE of the following questions:
Sketch Requirements: Draw a vertical section of the heart showing 4 chambers (RA, RV, LA, LV), major blood vessels (Aorta, Pulmonary Artery, Vena Cavae), and septum.
Labels for Valves:
- Tricuspid Valve: Between Right Atrium and Right Ventricle.
- Bicuspid (Mitral) Valve: Between Left Atrium and Left Ventricle.
- Pulmonary Semilunar Valve: At the base of Pulmonary Artery.
- Aortic Semilunar Valve: At the base of Aorta.
- Eustachian Valve: (At opening of IVC - usually vestigial).
- Thebesian Valve: (At opening of coronary sinus).
Function (Any one):
- Tricuspid Valve: Prevents the backflow of blood from the right ventricle into the right atrium during ventricular contraction.
HSK Pathway (Hatch-Slack Pathway / C4 Cycle):
This pathway occurs in C4 plants (e.g., Maize, Sugarcane) involving two types of cells: Mesophyll and Bundle Sheath.
- In Mesophyll Cell:
- CO2 is accepted by PEP (Phosphoenolpyruvate) in the presence of PEP carboxylase.
- Product: OAA (Oxaloacetic Acid - 4C compound).
- OAA is converted to Malic Acid (or Aspartic Acid).
- Transport: Malic acid is transported to Bundle Sheath cells.
- In Bundle Sheath Cell:
- Malic acid undergoes decarboxylation to release CO2 and Pyruvate.
- The released CO2 enters the Calvin Cycle (C3 cycle) to form glucose.
- Regeneration: Pyruvate is transported back to Mesophyll cells and regenerated into PEP using ATP.
Process Description:
- Approach of Sperm: Millions of sperms reach the ampulla. Capacitation prepares sperm for fertilization.
- Entry of Sperm (Acrosome Reaction): The acrosome releases lysins (Hyaluronidase) to penetrate the Corona Radiata and Zona Pellucida. The sperm head fuses with the oocyte membrane.
- Cortical Reaction: Upon entry of one sperm, cortical granules in the egg release enzymes that harden the Zona Pellucida, preventing polyspermy (fertilization membrane formed).
- Activation of Ovum: The entry stimulates the secondary oocyte to complete Meiosis II, releasing the second polar body and forming the female pronucleus.
- Syngamy (Fusion): The male pronucleus and female pronucleus fuse (Amphimixis) to form a diploid Zygote.
Diagrams required:
- Sperms attacking the ovum.
- Acrosome reaction and penetration.
- Extrusion of polar body and cortical reaction.
- Fusion of pronuclei.
(i) Cutting
(ii) Budding.
Artificial Vegetative Propagation: It is the process of growing new plants from vegetative parts of parent plants (root, stem, leaf) using man-made methods.
(i) Cutting:
- A small piece of any vegetative part of a plant with one or more buds is cut and planted in soil.
- Stem cutting: e.g., Rose, Sugarcane.
- Leaf cutting: e.g., Sansevieria.
- Root cutting: e.g., Blackberry.
(ii) Budding:
- It is a form of grafting where a single bud (scion) from a desired plant is inserted into a slit in the bark of a rooted stock plant.
- Common method: T-budding or Shield budding.
- Example: Rose, Orange, Peach.
The system associated with urine elimination is the Human Excretory System.
Components:
- Kidneys (Pair): Bean-shaped organs that filter blood to produce urine.
- Ureters (Pair): Muscular tubes that carry urine from the renal pelvis of the kidneys to the urinary bladder.
- Urinary Bladder: A muscular sac that temporarily stores urine. It has a smooth muscle layer called the Detrusor muscle.
- Urethra: A tube leading from the bladder to the exterior for the discharge of urine (micturition).
Diagram Labels Required: Kidney, Renal Artery, Renal Vein, Ureter, Urinary Bladder, Urethra.
--- End of Question Paper Solution ---